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Southern Blotting hybridization lambda DNA

Southern Blotting hybridization lambda DNA - Molecular Biology Techniques

Southern Blotting hybridization lambda DNA - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 03-04-2012, 07:26 AM
Pipette Filler
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Default Southern Blotting hybridization lambda DNA



There are a couples of question that i need help with...?

1. Based on your results, how many copies of probe sequence are present in the lambda genome?

2. How can you account for the faint bands that occur on the membrane in unexpected positions?

4. What factors influence the efficency of tranferring DNA restriction fragments to the membrane?

Thanks.
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blotting , dna , hybridization , lambda , southern


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