Can anybody tell me which, among the many types of monoclonal antibodies, should I use in order to catch, by flow cytometry, immunoglobulins from supernatants? Whole molecules or some chains? I can work only on 3 colours- with FITC, RPE-Cy5, PE.
(Another question, for which I haven't found an answer through almost 4 years, what kind of immunoglobulins can I find in supernatants after 72h and 120h of cell culture stimulation? I bet that I should have IgM and IgG at least.)
Or maybe ELISA-like kits are better? I saw one kit, 5 minutes of incubation, analyzed by spectrophotometer, but still don't know to price.