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#1
02-26-2012, 03:35 PM
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 flow cytometry anti-human anti-IgG,M,D,A,E Can anybody tell me which, among the many types of monoclonal antibodies, should I use in order to catch, by flow cytometry, immunoglobulins from supernatants? Whole molecules or some chains? I can work only on 3 colours- with FITC, RPE-Cy5, PE. (Another question, for which I haven't found an answer through almost 4 years, what kind of immunoglobulins can I find in supernatants after 72h and 120h of cell culture stimulation? I bet that I should have IgM and IgG at least.) Or maybe ELISA-like kits are better? I saw one kit, 5 minutes of incubation, analyzed by spectrophotometer, but still don't know to price. Please, help.  |
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| antihuman , antiigg , cytometry , elisa , flow , flow cytometry , immunglobulin , serum , supernatant |
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