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Troubleshoot Faint SDS-Page Bands with Blue Dye in Lane?

Troubleshoot Faint SDS-Page Bands with Blue Dye in Lane? - Molecular Biology Techniques

Troubleshoot Faint SDS-Page Bands with Blue Dye in Lane? - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 09-08-2011, 05:44 AM
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Default Troubleshoot Faint SDS-Page Bands with Blue Dye in Lane?



I am unable to get good bands in sds page i use 5% stacking gel and 12 % speparting gel, sample was denatured for 10 min at 90 oC satined in comassin blue for 30 min and destained overnight i have attached phot of gel 1st and last lane are markers lane 1,2,3,5,6,7 are protein samples 20 micro liters was loaded in biorad mini conatining 4x sample buffer diluted there is continues blue background in the lane i dont know why its is happening i ran at 60 volts constant for 3hrs aprox
please help me thank you
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