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how to attach dendritic cells for imaging?

how to attach dendritic cells for imaging? - Molecular Biology Techniques

how to attach dendritic cells for imaging? - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 08-03-2011, 07:47 AM
Pipette Filler
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Default how to attach dendritic cells for imaging?



Hi, I want to carry out actin staining on human dendritic cells derived from a cell line. The cells are grown in suspension. I am having trouble fixing the cells and subsequent staining because the cells do not attach to a surface (I have tried coating a glass slide with poly L lysine, but it didn't work). Are there any other ways to either attach the cells to a slide or to stain for actin in these cells? I will appreciate any help.

Thanks,
John.
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