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-   -   how to attach dendritic cells for imaging? (http://www.molecularstation.com/forum/molecular-biology-techniques/83287-how-attach-dendritic-cells-imaging.html)

aguy 08-03-2011 07:47 AM

how to attach dendritic cells for imaging?
 
Hi, I want to carry out actin staining on human dendritic cells derived from a cell line. The cells are grown in suspension. I am having trouble fixing the cells and subsequent staining because the cells do not attach to a surface (I have tried coating a glass slide with poly L lysine, but it didn't work). Are there any other ways to either attach the cells to a slide or to stain for actin in these cells? I will appreciate any help.

Thanks,
John.


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