i recently extracted proteins from a plant leaf, root and shoot and intended to analyze the proteins by SDS PAGE.
i prepared these samples by crushing the sample( i.e root, shoot, leaf) in extraction buffer, sterile distilled water and sample buffer. But i think i over diluted the samples. Once i ran SDS PAGE with these samples ( the maximum sample i can load is 30micro l)...i observed that the bands were tooo faint...and also there was a lot of trailing in lanes.
Could any 1 suggest ways to concentrate this already prepared sample so that i can get clear and distinct bands pleasee.