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agarose gel

agarose gel - Molecular Biology Techniques

agarose gel - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 05-13-2011, 06:48 PM
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Default agarose gel



hi guys
my DNA on agarose gel doesn't look very sharp (even marker, have this feathery effect), seems washed out". I usually run 80 V 1 TAE buffer. 1kb ladder seems ok, only problems with large DNA molecules :/ PCR products looks ok. Any ideas? TXC
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Old 06-15-2011, 04:37 AM
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Default Re: agarose gel

added EtBr to gel? Loading buffer? Gel concentration, keep in mind this effects your DNA resolution? From the way you say PCR producs are okay and your large DNA isn't, it sounds like you are stretching your gel's resolution. Solution: Seperate gels for PCR and large DNA.
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