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site-directed mutagenesis - primer purification

site-directed mutagenesis - primer purification - Molecular Biology Techniques

site-directed mutagenesis - primer purification - Molecular Biology Forum. Includes forums for common molecular biology techniques.

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Old 05-02-2011, 02:17 PM
Pipette Filler
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Default site-directed mutagenesis - primer purification

Hi all!

I have a question about purification of primers used in site-directed mutagenesis. In kit manual, there is a note: "primers ... must be purified either by fast polynucleotide liquid chromatography (FPLC) or by polyacrylamide gel electrophoresis (PAGE)". Bud most of companies producting primers offer purification by OPC or HPLC.
After brief googling I found out that OPC purification is used in site-directed mutagenesis. Is that right, or are there some reasonable differences in manuals between producers of kits so I should stand on HPLC purification?

Thanks a lot!
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mutagenesis , primer , purification , sitedirected

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