i am performing [of course not for the first time] northern blot, but i am stuck on one of the most basic step, that is control, i am using U6B as a normalizing control,
this is the probe sequence i am using, but unfortunately everytime i am getting nothing in the mature form region, here is the picture of blot i am getting,
i have performed this expression twise till now but still stuck with the same results. for your information, i am getting good bands at the mature region for other miRNA probes.
i will appreciate if anyone can suggest me something.
Thanks in advance.