| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| I am having trouble with one of the questions in my restriction digest assignment, a classmate and I have been thinking about it for days but we can't figure it out. Can anyone help? A large number of 10kbp plasmids are digested: Endonuclease / Fragments BamHI / 10 EcoRI / 10 PstI / 2.7 , 7.3 NotI / 10 SstI / 4.8 , 5.2 TaqI / 6 , 10 , 17 , 23 It then goes on to do double digests with varying combinations and I drew the circular restriction map. The 2nd part of the question asks to identify anomalies and say why they could happen. Obviously the anomaly is that somehow a 10kbp fragment is digested into 6, 10, 17, and 23 fragments. Also, for example, if you mix TaqI with PstI you get 2.7 and 7.3 fragments. This leads me to believe that the TaqI does not cut at all. But I have no idea whats going on with the 6, 10, 17, and 23. |
| Tags |
| assignment , difficulty , digest , restriction |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Problem of time required for complete digestion using Fast digest enzymes | srivels | Molecular Cloning Forum | 1 | 01-25-2010 06:49 PM |
| restriction enzyme to digest 16srRNA pcr product | Duncan Clark | Microbiology Forum | 0 | 08-04-2009 03:44 PM |
| Yeast Digest, Vol 45, Issue 2 | Shaoyu Wang | Yeast Forum | 0 | 02-05-2009 06:09 PM |
| Restriction analysis software | aftabac | Bioinformatics | 2 | 07-05-2008 01:35 AM |
| Restriction Digest Protocol Lab for Students. | domba | Molecular Biology Articles and Protocols | 0 | 07-31-2006 11:11 PM |