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Dissolving a chemical

Dissolving a chemical - Molecular Biology Techniques

Dissolving a chemical - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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  #1  
Old 10-12-2010, 09:19 AM
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Question Dissolving a chemical



I want to ask,
Is there is any difference between

1. to dissolve a chemical directly in 1000ml water

2. dissolving a compound in little quantity of water and then making its volume upto 1000ml.

is it cause any change???

thanks...
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Old 10-13-2010, 06:05 PM
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Default Re: Dissolving a chemical

Yes it does. The chemical will raise the volume of the solution and it won't be 1000 ml anymore, which ultimately change the final concentration of the chemical in the solution.

The best way to prepare a solution with a know concentration of a certain chemical (powder) is to weight the needed amount of the chemical and then dissolve it in a no more than 80% of the total volume of solvent (water, etOH or whatever). Once your chemical has completely dissolved, raise the volume to, in this case, 1000 ml.
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Old 10-15-2010, 03:18 PM
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Smile Re: Dissolving a chemical

Quote:
Originally Posted by luisillo View Post
Yes it does. The chemical will raise the volume of the solution and it won't be 1000 ml anymore, which ultimately change the final concentration of the chemical in the solution.

The best way to prepare a solution with a know concentration of a certain chemical (powder) is to weight the needed amount of the chemical and then dissolve it in a no more than 80% of the total volume of solvent (water, etOH or whatever). Once your chemical has completely dissolved, raise the volume to, in this case, 1000 ml.
The post above is absolutely correct.
Some dunces tend to disagree when the subject comes up, because they don't understand the concept of mass.
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