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cloning Fab fragment from hybridoma

cloning Fab fragment from hybridoma - Molecular Biology Techniques

cloning Fab fragment from hybridoma - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 07-02-2010, 10:49 PM
Pipette Filler
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Default cloning Fab fragment from hybridoma





I have two hybridomas that secrete IgMs. I want to clone the Fab fragment from both, but I do not have experience with antibodies. Is there someone who can give me some pointers regarding primer design? I suppose there may be standard sets of primers to try, but I could not find useful info in the literature.
Should I expect the affinity of the Fab fragment to the antigen to decrease compared to the IgM?
Any help is very much appreciated!!
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