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About rna and cdna

About rna and cdna - Molecular Biology Techniques

About rna and cdna - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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  #1  
Old 06-15-2010, 05:37 AM
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Default About rna and cdna



hi to all and thanks for accepting me in your forum.My question is that if we are getting good 260/280 cDNA values but no bands on agarose gel electrophoresis what might be the reason please sugest.





With Regards,
supinder
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Old 06-15-2010, 08:58 PM
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Default Re: About rna and cdna

Can you run other gels successfully? If not, check whether you're running it backwards or have forgotten to include ethidium bromide.
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supinder (06-16-2010)
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Old 06-16-2010, 06:44 AM
Pipette Filler
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Default Re: About rna and cdna

Quote:
Originally Posted by Bonnie View Post
Can you run other gels successfully? If not, check whether you're running it backwards or have forgotten to include ethidium bromide.
THANKS but I have included ethidium bromide what are the suitable values of 260/280 for cDNA as we are isolating RNA by Trizol method again thanks for your help
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