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How to find the mechanism on differential gene expression
We have been doing research on the differential expression of one gene between two cell lines (A and B cell lines), with high expression in A cell line and no expression in B cell line. We found that ~250 bp in the promoter of the gene has its activity in A cell line, but no activity in B cell line. So we think the differential profiling of transcriptional factors between A band B cell lines decide whether the gene is expressed. However, I donít know to find the transcriptional factors regulating the expression of the gene. Please give me some suggestions.
Re: How to find the mechanism on differential gene expression
Did you try to search for consensus for transcription factor within your 250bp? You can do that by using transfact .
|differential , expression , find , gene , mechanism|
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