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How to find the mechanism on differential gene expression

How to find the mechanism on differential gene expression - Molecular Biology Techniques

How to find the mechanism on differential gene expression - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 02-10-2010, 04:16 PM
Pipette Filler
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Default How to find the mechanism on differential gene expression



We have been doing research on the differential expression of one gene between two cell lines (A and B cell lines), with high expression in A cell line and no expression in B cell line. We found that ~250 bp in the promoter of the gene has its activity in A cell line, but no activity in B cell line. So we think the differential profiling of transcriptional factors between A band B cell lines decide whether the gene is expressed. However, I donít know to find the transcriptional factors regulating the expression of the gene. Please give me some suggestions.
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Old 02-11-2010, 09:13 PM
Pipette Filler
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Default Re: How to find the mechanism on differential gene expression

Hi,
Did you try to search for consensus for transcription factor within your 250bp? You can do that by using transfact .
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