Originally Posted by HTS76
What is normalization of data and why do we care for it?
If you want to understand why normalization is done for PCR assays, have a look at
Applications Guide' from Bio-Rad, i have this PDF but don't know its html...sorry but you will need to snoop around this, start at the biorad website. I can send it to you if you give me your email. Basically it controls for experimental variance
i.e efficiency of cDNA extraction vary in different samples ( say cancer patient 1,2 etc )of same group ( say cancer) as well as controlling (normalizing better term) for biological variance
( maybe patient 1 >expression of PCR target slightly more relative to patient 2)
Usually these genes you normalize to are similar in expression across the groups ( i.e cancer vs non-cancer) , GAPDH and B-actin are some common ones referred to as reference genes.
"Critical Factors for Successful Real-Time PCR - English (PDF)"
QiAgen websites : Literature> Brochures & Application Guide
Sorry can't post the link as im <5 post