| | Re: ACEMBL system
ACEMBL is a 3rd generation multi-gene expression system for complex production in E. coli, created at the European Molecular Biology Laboratory EMBL, at Grenoble.
ACEMBL can be applied both manually and also in an automated setup by using a liquid handling workstation. ACEMBL applies tandem recombination steps for rapidly assembling many genes into multigene expression cassettes. These can be single or poly-cistronic expression modules, or a combination of these elements.
ACEMBL also offers the option to employ conventional approaches involving
restriction enzymes and ligases if desired, which may be the methods of choice in laboratories not familiar with recombination approaches.
The following strategies for multigene assembly and expression are provided
for in the ACEMBL system and detailed in Sections B and C:
(1) Single gene insertions into vectors (recombination or restriction/ligation)
(2) Multigene assembly into a polycistron (recombination or restriction/ligation)
(3) Multigene assembly using homing endonucleases
(4) Multigene plasmid fusion by Cre-LoxP reaction
(5) Multigene expression by cotransformation
These strategies can be used individually or in conjunction, depending on the project and user.
ACEMBL is distributed by ATG:biosynthetics GmbH