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What happens during the process of conjugating BSA to oligos and BSA to SANH?

What happens during the process of conjugating BSA to oligos and BSA to SANH? - Molecular Biology Techniques

What happens during the process of conjugating BSA to oligos and BSA to SANH? - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 09-23-2009, 10:06 PM
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Default What happens during the process of conjugating BSA to oligos and BSA to SANH?



What happens during the process of conjugating BSA to oligos and BSA to SANH? In other words, what is the science behind it?

Okay, so I'm doing a research report. I followed a protocol for the modification of BSA. I want to see if conjugating BSA to oligos probes will increase the sensitivity of the results in my DNA microarray chip. So far, I conjugated BSA to oligos powder and also conjugated BSA to SANH. Since oligos is relatively small and BSA is a large protein, I hoped to increase the sensitivity. After running a protein gel, I saw that the well that contained BSA conjugated to the oligos shifted upwards compared to the well that had BSA conjugated to SANH.
Therefore, I can conclude that the oligos did get larger after conjugating with the BSA, and that they did bind. Hopefully, this will increase the sensitivity of my results when I conjugate BSA to the oligos probes on the DNA chip. (By the way, we are designing DNA chips for efficiently genotyping HPV strains.)
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Old 10-16-2009, 12:44 AM
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Default Re: What happens during the process of conjugating BSA to oligos and BSA to SANH?

Thanks for sharing these info with us!
I will keep in touch with your blogreading..
Stumbled your URL…
have a great day
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bsa , conjugating , conjugation , dna chip , oligonucleotides , oligos , process , sanh


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