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Flow cytometry basic question urgently

Flow cytometry basic question urgently - Molecular Biology Techniques

Flow cytometry basic question urgently - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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  #1  
Old 08-31-2009, 05:45 AM
Pipette Filler
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Question Flow cytometry basic question urgently



Can we detect a surface marker (by surface staining) and intracellular cytokine (by permeabilization) for a certain cell population at the same time by flow cytometry?
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Old 09-01-2009, 02:12 PM
Pipette Filler
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Default Re: Flow cytometry basic question urgently

Yes, I think it is possible.
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Old 09-02-2009, 07:28 AM
Pipette Filler
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Default Re: Flow cytometry basic question urgently

It should be possible - I have certainly done concurrent extracellular and intracellular stains for flow cytometry before. It tended to work better if I did the extracellular stain first, then permeabilize and do the intracellular stain.
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  #4  
Old 09-02-2009, 07:51 AM
Pipette Filler
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Default Re: Flow cytometry basic question urgently

I thank you for your reply cause yesterday one of my friends gave me a reference saying the same. Thanks for sharing your experience.
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