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#1
08-31-2009, 05:45 AM
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 Flow cytometry basic question urgently Can we detect a surface marker (by surface staining) and intracellular cytokine (by permeabilization) for a certain cell population at the same time by flow cytometry?  |
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#3
09-02-2009, 07:28 AM
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 Re: Flow cytometry basic question urgently It should be possible - I have certainly done concurrent extracellular and intracellular stains for flow cytometry before. It tended to work better if I did the extracellular stain first, then permeabilize and do the intracellular stain. |
| The Following User Says Thank You to quack For This Useful Post: | ||
altawfik (09-02-2009)
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#4
09-02-2009, 07:51 AM
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| Re: Flow cytometry basic question urgently I thank you for your reply cause yesterday one of my friends gave me a reference saying the same. Thanks for sharing your experience. |
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| basic , cytometry , flow , question , urgently |
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