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Trouble with Fractionation of ER and Golgi

Trouble with Fractionation of ER and Golgi - Molecular Biology Techniques

Trouble with Fractionation of ER and Golgi - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 07-27-2009, 04:01 AM
Pipette Filler
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Default Trouble with Fractionation of ER and Golgi



This technique seems really straightforward from literature, but unfortunately, I've been struggling to achieve acceptably enriched fractions. I'm currently using rat brain and intestine -- the latter is the control -- to study the molecular forms of acetylcholinesterase along the secretory pathway. Also, I'm using a 10-30% continuous OptiPrep gradient, but end up achieving ER spread throughout. Any help would be greatly appreciated. Thanks!
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