queries

[neha.gupta]

hello all
i m new to use PAGE.....i want to know that when we can use acrylamide for separation of dna and when to use agarose gel electrophoresis???

secondly,can RNase degrade dna???if yes...how & why????

lastly i want to know that if have 2 gels running dna and one stained with methylene blue show no bands and the one gel having same dna sample is stained with ethidium bromide shows bands in uv light.why????????

[jiajia1987]

Acrylamide is useful when you want to separate DNA that differs by a single basepair in length. Agarose gel electrophoresis is used when your DNA differs by a longer length, eg, 200bp or so. Both can be used to check your PCR products or whatever. It is just that their resolution is different. That's what I know.

RNase, as its name implies, degrades RNA, not DNA. It is an enzyme that chops up RNA.