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Recombinant proteins refolding!

Recombinant proteins refolding! - Molecular Biology Techniques

Recombinant proteins refolding! - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 06-05-2009, 03:09 AM
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Default Recombinant proteins refolding!



Hi!
I work with plant lectin expression in E.coli.
I get the most part of the proteins from the insoluble fraction, as inclusion bodies, but, when I take off the denaturant the protein precipitate.

I'm looking for efficients refolding protocols to help me in my research.

I appreciate any tip!

Thanks a lot
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