I am presently attempting to isolate and detect a DNA band from a short sequence oligonucleotide 8 bases long using PAGE electrophoresis.
Albert's Molecular Biology of the Cell book recommends sequence size range for PAGE of 10 - 500 bucleotides, would 8 nucleotides sequence still work with PAGE? If it would work, any additional useful hints concerning pocedure and protocol beyond the standard PAGE procedures would be appreciated.
Also is there a standard DNA ruler for very short DNA sequences?
I thank you for your kind assistance!