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Short Sequence Nucleotides Electrophoresis

Short Sequence Nucleotides Electrophoresis - Molecular Biology Techniques

Short Sequence Nucleotides Electrophoresis - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 03-18-2009, 06:17 AM
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Default Short Sequence Nucleotides Electrophoresis



I am presently attempting to isolate and detect a DNA band from a short sequence oligonucleotide 8 bases long using PAGE electrophoresis.

Albert's Molecular Biology of the Cell book recommends sequence size range for PAGE of 10 - 500 bucleotides, would 8 nucleotides sequence still work with PAGE? If it would work, any additional useful hints concerning pocedure and protocol beyond the standard PAGE procedures would be appreciated.

Also is there a standard DNA ruler for very short DNA sequences?

I thank you for your kind assistance!
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Old 03-18-2009, 10:37 PM
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Default Re: Short Sequence Nucleotides Electrophoresis

should work. if your sample is basically one band you can run it with a marker (look for 10bp ladder) and stop the gel before the sample runs off, (probably 60-70% of the way).
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