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#1
03-18-2009, 06:17 AM
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 Short Sequence Nucleotides Electrophoresis I am presently attempting to isolate and detect a DNA band from a short sequence oligonucleotide 8 bases long using PAGE electrophoresis. Albert's Molecular Biology of the Cell book recommends sequence size range for PAGE of 10 - 500 bucleotides, would 8 nucleotides sequence still work with PAGE? If it would work, any additional useful hints concerning pocedure and protocol beyond the standard PAGE procedures would be appreciated. Also is there a standard DNA ruler for very short DNA sequences? I thank you for your kind assistance!  |
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#2
03-18-2009, 10:37 PM
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| Re: Short Sequence Nucleotides Electrophoresis should work. if your sample is basically one band you can run it with a marker (look for 10bp ladder) and stop the gel before the sample runs off, (probably 60-70% of the way). |
| The Following User Says Thank You to danfive For This Useful Post: | ||
admin (03-19-2009)
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| electrophoresis , nucleotides , sequence , short |
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