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Short Sequence Nucleotides Electrophoresis
I am presently attempting to isolate and detect a DNA band from a short sequence oligonucleotide 8 bases long using PAGE electrophoresis.
Albert's Molecular Biology of the Cell book recommends sequence size range for PAGE of 10 - 500 bucleotides, would 8 nucleotides sequence still work with PAGE? If it would work, any additional useful hints concerning pocedure and protocol beyond the standard PAGE procedures would be appreciated.
Also is there a standard DNA ruler for very short DNA sequences?
I thank you for your kind assistance!
Re: Short Sequence Nucleotides Electrophoresis
should work. if your sample is basically one band you can run it with a marker (look for 10bp ladder) and stop the gel before the sample runs off, (probably 60-70% of the way).
|The Following User Says Thank You to danfive For This Useful Post:|
|electrophoresis , nucleotides , sequence , short|
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