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Streaking out on agar plates

Streaking out on agar plates - Molecular Biology Techniques

Streaking out on agar plates - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 02-24-2009, 06:05 AM
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Default Streaking out on agar plates



I am having trouble streaking out. I am working with contaminated cultures of Cronobacter sakazakii and am supposed to create pure cultures. So I need to get single colonies on my plates but the organism is growing like a spreader, creating a kind of layer on the agar and do not produce any distinguishable colonies at all. If any one has an idea about whats going on, plz let me know
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Old 02-24-2009, 10:07 AM
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Default Re: Streaking out on agar plates

You may add motility inhibiting substances to your agar base. These depend on the microorganism, but in cases of Pseudomonas and Enterobacter this may be addition of NaCl or novobiocin. Antibiotics may inhibit bacterial motility, depending on how motility is controlled on molecular level. Of course the antibiotics you choose can't totally inhibit the growth of your bacteria.

Try to look at the references - you may find some hints in articles.
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Old 02-25-2009, 02:30 AM
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Default Re: Streaking out on agar plates

if you can afford to buy or ask for sample for this media [Only registered users see links. ]
[Only registered users see links. ]
**this journal did mention presence of FDA method for its detection... maybe u could have a look there

Last edited by butters; 02-25-2009 at 02:31 AM. Reason: spelling error
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