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Help with Electrolytic Dissociation

Help with Electrolytic Dissociation - Molecular Biology Techniques

Help with Electrolytic Dissociation - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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  #1  
Old 12-06-2008, 05:58 PM
Pipette Filler
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Exclamation Help with Electrolytic Dissociation



Hey, im a pre IB idiot who decided to do a project on Svante Ahrennius electrolytic theory of dissociation
and Werner Heisenbergs :"creation of quantum mechanics, the application of which has, inter alia, led to the discovery of the allotropic forms of hydrogen"

neither of which i know nothing about
im extremely greatful for any help!!!
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Old 12-17-2008, 09:32 AM
Pipette Filler
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Default Re: Help with Electrolytic Dissociation

Hi, I am doing a dot blot assay on nitrocellulose membrane, and it works well with commercial proteins, but it doesn't work when I try it with self-made recombinant proteins. Our proteins are expressed in E. coli and are purified with Ni/NTA-purification columns and eluted with a buffer containing 6M urea. Any suggestion why this doesn't work is welcome.
Thanks!
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Old 07-17-2009, 02:13 AM
Pipette Filler
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Default Re: Help with Electrolytic Dissociation

Hello everyone. Nice to meet you all.
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dissociation , electrolytic


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