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| I would like to ask if anyone has checked whether there is a "control gene" (Q-RT-PCR) to show that the demethylating drug 5'Aza is indeed working. I know it is rather difficult to have a so-called universal "control gene" due to different cell types may have different methylation patterns. And I notice that most papers do not have this control. Please cite the "references" for such control gene. Many thanks! HsinPai |
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| 5aza , control , treatment |
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