No cell growth with antibiotic plates

[hdraheim]

I have recently poured plates with 1) 50ug/ml ampicillian 2) 50 ug/ml kanamycin and 3) with no antibiotics. After transformation and plating the only plates that grew colonies were the ones with no antibiotics. Also, despite using blue/white screening all colonies were white?
Does anyone know what went wrong here?

Thanks,
Hope

[kmunson779]

Re: blue-white screening -- did you did add IPTG and X-Gal to those plates?

Do you have a positive transformation control (an intact plasmid with an antibiotic resistance gene)? I guess I should say, which controls did you run?

Does your no antibiotic plate have separated colonies or a lawn? I would expect a lawn if your competent cells are okay.