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Extracting HDAC 2 enzyme from E.coli cells
Hi people, I'm new here. Currently a student.
I have been given this problem of extracting the HDAC 2 enzyme from E.coli before testing if the enzymes expressed are working (active). May I know how should I go about doing it? What are the things that need to be considered?
For cell disruption, there are many ways. Enzymatic, chemical, physical. Which is the best? the typical protocol which uses lysosyme?
After which, do I need to take into account if inclusion bodies are formed?
|cells , ecoli , enzyme , extracting , hdac|
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