Proteinase K mechanism

[bam pandit]

hellossss peoplesssss!!!!!

i'm curious to know the mechanism of Proteinase K 's action, so that i have a gist of how it can be exploited in various experiments...

humble request: please do not post what you think, post what you know

[bam pandit]

also, what does the K stand for and whats its significance?? ..... this one should be dumb... !!!

[molecule2005]

Hello Interesting question.

I have used Proteinase K, and it digests every protein! Proteinase K is a broad acting serine protease. Proteinase K was discovered in 1974 from extracts of the fungus Tritirachium album.

Here is the original article:
Ebeling W, Hennrich N, Klockow M, Metz H, Orth HD, Lang H (1974). "Proteinase K from Tritirachium album Limber". Eur. J. Biochem. 47 (1): 91–7. PMID 4373242.


If you need more info check here:
ENZYME entry 3.4.21.64

Not sure why it was called "K" but the original article may have a clue.

[bam pandit]

i went through that article before. it'll tells you the properties of Proteinase K, but the paper doesn't mention anything about the mechanism apart from the the involvement of serine in the mechanism.
however, the article is very useful. thanks for the help.