When you mean "cured" you mean removal of the plasmid?
The definition I know is: curing is the elimination
of a plasmid from its host cell.
If that is so, I found this protocol which was from an
old bacteriologist and it worked for a friend of mine
(who did it once though):
- Grow bacteria with plasmid overnight. use approximately 2x10^9 cells/ml.
- Dilute them to 1x10^4 cells/ml in LB, 0.1M Tris, pH 7.6, 20ug/ml acridine orange.
- Grow overnite in dark.
- Replica plate to find "cured" bacteria.
Also, you can use Novobiocin as it interferes with gyrase
(as does Nalidixic acid and several other agents -
Oxolinic Acid) which is needed for plasmid replication.
ethidium bromide has been used along with other intercalating
see this paper for a new method: [Only registered users see links. ]
It also helps to culture the bacteria at higher temperatures such
as 42C or 45C.
See this paper for more information on temperatures: [Only registered users see links. ]