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Introduction of foreign DNA by transposition

Introduction of foreign DNA by transposition - Molecular Biology Techniques

Introduction of foreign DNA by transposition - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 11-29-2007, 09:37 PM
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Default Introduction of foreign DNA by transposition



I am currently reading up on the use of transposition to introduce foreign DNA into a eukaryotic genome, and the only examples I have found are the use of P-elements to introduce DNA into the germline of drosophila (Rubin and Spradling), and the maize Ac element (McClintock) for introduction into various plant species. The P-element example obviously does not have many applications, but the plant Ac element seems to have a wider application. Sould I be content with these main examples, or are there others? (Personally I find it hard to gather the enthusiasm to study plants!)
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Old 11-30-2007, 01:10 AM
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Default Re: Introduction of foreign DNA by transposition

Check out RMCE in wikipedia ---> [Only registered users see links. ]

Excerpt:
"In the field of reverse genetics RMCE (recombinase-mediated cassette exchange) is of increasing relevance. The procedure permits the systematic, repeated modification of higher eukaryotic genomes by targeted integration. For RMCE, this is achieved by the clean exchange of a preexisting "gene cassette" for an analogous cassette carrying the "gene of interest" (GOI)."
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