Long Terminal Repeats (LTR)

SRD1 · #1 (**permalink**) 11-20-2007, 09:47 AM

Hello,

I was hoping someone would be able to advise me on how to identify long terminal repeats. I'm in the process of designing primers to screen gDNA from clones which (hopefully) will contain a knockout. This is an alternative to Southern blotting (which has not been good to me!) I've been warned the region that I'm designing my forward primer will contain an LTR. I'm not sure how I can find where this starts and so avoid including it in the primer sequence. Are there any databases or search tools on the web I can use for this? I've tried downloading the "LTR-STRUCT" application but I keep generating error reports.

Thanks

Sally

danfive · #2 (**permalink**) 11-20-2007, 02:12 PM

If you have the sequence you should recognize the repeats once you see them.

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Long Terminal Repeats (LTR)

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