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Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA
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Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA

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Old 11-19-2007, 08:23 PM
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Default Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA
hello fellas,
here to look for some gud protocols for gel-shift assays (non-radioactive).
any help is appreciated..
thanks
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Old 01-23-2008, 03:50 AM
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Default Re: Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA
Hello,
there are many techniques for non radioactive EMSA.

I have used biotin-UTP for RNA EMSA, however you can use biotin-CTP for DNA EMSA.

The light shift kit from Pierce is quite good.

Please let use know how you do/which protocol you choose.

Cheers!
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