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Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA

Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA - Molecular Biology Techniques

Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 11-19-2007, 09:23 PM
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Default Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA



hello fellas,
here to look for some gud protocols for gel-shift assays (non-radioactive).
any help is appreciated..
thanks
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Old 01-23-2008, 04:50 AM
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Default Re: Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA

Hello,
there are many techniques for non radioactive EMSA.

I have used biotin-UTP for RNA EMSA, however you can use biotin-CTP for DNA EMSA.

The light shift kit from Pierce is quite good.

Please let use know how you do/which protocol you choose.

Cheers!
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Old 01-06-2009, 05:32 AM
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Default Re: Non-Radioactive Protocols for Gel-Mobility Shift Assays EMSA

I checked the kit and it seems that it's designed for DNA-protein interaction. How did you use it for RNA-protein binding? Do you have a modified protocol or you just followed the manufacturer's manual? The kit supplys biotin-11-UTP even for DNA. Is is applicable to RNA? If it's also good for RNA, I wanna try the kit. Many thanks!
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assays , emsa , gelmobility , nonradioactive , protocols , shift


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