Methods for gene/protein identification and function

[koojo]

Hello, I'm writing a 'mach' grant proposal for one of my advanced bio courses. I answering some answered question from a journal that was written about 2 co-expressed genes in D. melanogaster that are used for CO2-sensitivity. However, the article did not specify as to the individual physiological roles of each gene in CO2 recognition. They did mention that their previous attempts to tag these two proteins while retaining function have failed. However, possible roles proposed include one subunit acting as a chaperon co-receptor while the other acts to differentiate between ligands, or possibly both subunits are required for both functions.

Therefore, I need some possible methods of gene tagging to determine the roles of these two gene, while retaining their function.
Genes: Gr21a and Gr63a

I did find a method called 'split green' to tag proteins, but I need some more. ANY help will be appreciated. Thank you.

[koojo]

Anyone? I'm basically looking for a method to use to figure out how the subunits function.

[danfive]

Check out the methods in this paper: Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration, Brian Stramer et al. link ---> http://www.jcb.org/cgi/content/full/168/4/567

The investigators tagged two proteins with GFP and created homozygous lines, then used cinemicroscopy to video the protein pathway/movement/localization.

[koojo]

Great!! Thanks for the input I will check it out.