Site-specific GFP Targetting

sotiri · #1 (**permalink**) 11-07-2007, 05:38 PM

Dear Colleagues,

I am trying to find a way to target the GFP gene to a specific locus/gene in a human embryonic stem cell line. Could you please advise me what the best way to do this is? Is there a commercial kit available for this application? Thank you.

danfive · #2 (**permalink**) 11-07-2007, 06:35 PM

You may want to search for "Recombinase-Mediated Cassette Exchange (RMCE) method to perform site-specific chromosomal integration."

danfive · #3 (**permalink**) 11-07-2007, 07:30 PM

Check out RMCE in wikipedia (excellent article) --> http://en.wikipedia.org/wiki/Recombi...sette_exchange

sotiri · #4 (**permalink**) 11-07-2007, 07:55 PM

Very interesting article! Thanks!

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Site-specific GFP Targetting

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