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· · · RNA Pictures 7 photos 6 comments |
· · · RNA Pictures 7 photos 6 comments |
· · · RNA Pictures 7 photos 6 comments | |||
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09-26-2007, 08:35 PM
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 DNA Pol III and Processivity How did O'Donnell show that the sliding clamp complex actually slides along dsDNA providing processivity to DNA Pol III? I think he took dna, a primer, and one radioactive dNTPs, added Pol III that was isolated, ran a gel, and the conclusion was that it was processive because on the gel, the distributive section had many bands and processive had one band. I dunno. does anyone know what the experiment was called and what the hypothesis was?        |
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11-04-2007, 12:07 PM
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 Re: DNA Pol III and Processivity Hello, not sure which article but I found: this recent paper about the Sliding Clamp Model: http://www.jbc.org/cgi/content/abstract/M211741200v1 and a nice paper here too: http://www.pubmedcentral.nih.gov/art...?artid=1171170 Total reconstitution of DNA polymerase III holoenzyme reveals dual accessory protein clamps http://www.jbc.org/cgi/content/abstract/265/2/1179 |
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