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Am currently having a problem in Western Blotting. Am investigating the expression of HIF-1alpha,in Insulinoma cells produced from Rabbit. The primary antibody am using is labelled "Protein G-purified Rabbit Anti-Human/mouse HIF-1alpha antibody and my secondary antibody labelled "Anti Rabbit IGn/HRP". My dilemma is if the absence of bands on my film be due to the fact that my cells are of Rabbit origin while the primary antibody is directed against "human/mouse" even though it was purified in Rabbit. Should it be "uman/Rabbit"then? All comments are welcomed.