Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Articles and Protocols
Register Search Today's Posts Mark Forums Read

Molecular Biology Articles and Protocols Post Molecular Biology and science Protocols, Reviews, and Articles Forum. You can be the author!


regarding primer designing

regarding primer designing - Molecular Biology Articles and Protocols

regarding primer designing - Post Molecular Biology and science Protocols, Reviews, and Articles Forum. You can be the author!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-07-2007, 08:41 AM
Pipette Filler
Points: 1,486, Level: 22 Points: 1,486, Level: 22 Points: 1,486, Level: 22
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2007
Location: hyderabad
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default regarding primer designing



hi all,

happy to be as a member of this station. i am here with some doubts regarding primer designing. please help me.

what is dG value given by the oligo analyser
what should be the optimum value of dG

thanks in advance
bye
priya
Reply With Quote
  #2  
Old 02-08-2007, 05:02 PM
Pipette Filler
Points: 1,425, Level: 22 Points: 1,425, Level: 22 Points: 1,425, Level: 22
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: regarding primer designing

le protocole et les mecanismes
Reply With Quote
  #3  
Old 02-09-2007, 08:07 AM
moleculardude's Avatar
M.D/Ph.D
 
Join Date: Jul 2006
Location: Melbourne Australia
Posts: 423
Thanks: 5
Thanked 10 Times in 8 Posts
Default Re: regarding primer designing

Hello there Priya!

and welcome to the forums:

dG is the free energy (G) value of the entire oligonucleotide.

I wouldn't worry about dG values between the two oligonucleotides.
Reply With Quote
  #4  
Old 05-15-2007, 05:25 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: regarding primer designing

Hi Priya,

the lesser the dG, bette it is. A low value will indicate that the bonds between the base pairs in oligos will break easily and won't interfere in the progress of a PCR reaction. Higher dG means you need to run PCR at a higher temperature
Reply With Quote
  #5  
Old 05-18-2007, 06:26 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: regarding primer designing

The dG calculations do not matter at higher Tm values
Reply With Quote
  #6  
Old 05-18-2007, 06:26 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: regarding primer designing

but specificity is one very important matter to be taken into account
Reply With Quote
  #7  
Old 05-18-2007, 06:27 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: regarding primer designing

And may be primer efficiency as well. You need to see if the template on which primers are being designed is OK and doen't folds upon itself. You know self complementarity
Reply With Quote
Reply

Tags
designing , primer


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Primer designing for expression vector yoginee budhkar Protocols and Methods Forum 0 09-16-2009 01:29 PM
Help in Primer designing for expression... Shri Protocols and Methods Forum 3 09-12-2009 07:03 PM
I need immediate help!! How to PCR primer designing? :help: veniesh PCR - Polymerase Chain Reaction Forum 2 08-20-2009 04:00 PM
Help in Primer designing for expression... shifalichatrath Protocols and Methods Forum 0 07-20-2009 07:12 AM
Real time SYBR PCR primer designing tarun gupta Protocols and Methods Forum 0 07-13-2006 06:22 AM


All times are GMT. The time now is 02:31 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14569 seconds with 16 queries