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Plasma cells in circulating blood.

Plasma cells in circulating blood. - Molecular Biology Articles and Protocols

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  #11  
Old 10-26-2012, 03:03 PM
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Default Re: Plasma cells in circulating blood.

I don't use LPS. I did few tests and I found in some people's supernatant IgG. When I finish I will have to confront the antibodies titres with MFI of surface antigens (among others I evaluated CD40 and CD40L). This is just crazy.
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  #12  
Old 10-27-2012, 06:40 AM
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Default Re: Plasma cells in circulating blood.

Good luck
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Old 03-13-2013, 01:29 PM
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Default Re: Plasma cells in circulating blood.

Is this possible that I after culture there is higher concentration of IgG than IgM? The difference is not significant, but I found it.
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Old 03-13-2013, 01:33 PM
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Default Re: Plasma cells in circulating blood.

After 72 hours I didn't find IgG, but after 120h I found and it some cases it was higher than IgM, but maybe this is normal, coz IgM is fast response and IgG is after switch?
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  #15  
Old 03-13-2013, 03:22 PM
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Default Re: Plasma cells in circulating blood.

I've never done it but, according to literature (I don't have the article right now, though), IgG appears in long term cultures: 5 or more days. The authors mentioned something about not changing media enhances IgG over IgM production. I will try to get at least the reference and post it.

Good luck
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Old 03-13-2013, 03:34 PM
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Default Re: Plasma cells in circulating blood.

Might help you:

[Only registered users see links. ]
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Old 03-13-2013, 03:34 PM
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Default Re: Plasma cells in circulating blood.

O, thanks.
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Old 03-17-2013, 02:12 PM
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Default Re: Plasma cells in circulating blood.

I am reading now article about PBMC stimulation with Pokeweed (PWM) + S. aureus + CpG. They are tracking in these research memory B cells and counting IgG titres, so as I understand such a stimulation activated memory B cells. Since 40% of B cells in circulating blood are memory B cells then I also have them in my culture, BUT I stimulate T cells with anti-CD3 and due to cytokine production and also CD40-CD40L (etc.) interaction I activate B cells. Is anti-CD3 stimulation able to stimulate memory B cells? i dont add any specific antigen to provoke memory B cells. I activate only T cells.
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Old 03-17-2013, 02:34 PM
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Default Re: Plasma cells in circulating blood.

I read at mirorao.com that memory B cells can be activated only by protein antigen which I dont have in my culture. So IgM and IgG titres I received appeared from newly formed plasma cells which were generated after anti-cd3 stimulation of T cells. There are almost no plasmablasts and plasmocytes in blood of healthy humans so they were created de novo in my culture. Am I finally right?
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blood , cells , circulating , immunology , memory cells , plasma , plasma cells , plasmablasts


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