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12-11-2006, 10:52 AM
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 Help  please I loock for a protocol for protein extraction from plant for FPLC analysis! Also protocol for RFLP, or AFLP or RAPD!!!? Think's
__________________  Walid ELFALLEH PhD Student Institut des Régions Arides (IRA) Rue Djorf - Km 22.5- 4119 Médenine Tunisie Web: http://www.ira.rnrt.tn E-mail: lwalidl@hotmail.com Phone :+216 75633005 Fax : +216 75633006        |
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04-17-2008, 05:35 PM
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 Re: Help for RAPD analysis use the following: Taq 1U dNTPs 200 uM Primer 20 pMol Buffer 1 x MgCl2 2.5 mM DNA 25-50 ng (according to your material) for PCR 94 C - 4 min 94 C- 1 min 37 C - 1 min 72 C - 2 min 72 C - 10 min Megha (Ph. D. student) |
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