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Microstudent 03-06-2014 09:38 AM

Microbiology Help [spread plates and biochemical tests on metabolism]
 
I have a few questions I am unable to answer:
I have just started studying Microbiology.

Other than allowing agar plates to dry to avoid bacteria running together why would you spread 0.1ml onto the surface of an agar plate when preforming viable counts.

This next one has me really stuck - we haven't covered anything like this..

Biochemical tests are used to help identify microorganisms based on their metabolic reactions. What are the main limitations of these methods?
Which method is now generally preferred and why?

Cheers

butters 03-07-2014 02:52 AM

Re: Microbiology Help [spread plates and biochemical tests on metabolism]
 
Answer are based on my microlab experiment (very limited)

Quote:

Originally Posted by Microstudent (Post 448141)
I have a few questions I am unable to answer:
I have just started studying Microbiology.

Other than allowing agar plates to dry to avoid bacteria running together why would you spread 0.1ml onto the surface of an agar plate when preforming viable counts.
Is this the full question or does it have other sentencies prior this?

This next one has me really stuck - we haven't covered anything like this..

Biochemical tests are used to help identify microorganisms based on their metabolic reactions. What are the main limitations of these methods?
Single colony, no mixture of different bacteria in the inoculum
Does bacteria grow on such media?
If the bacteria is still viable.


Which method is now generally preferred and why?
Prefer method: Polymerase Chain Reaction PCR or related RT PCR etc etc...

Cheers

Please do seek out other proper phrase or example, Cheer and TGIF!

Microstudent 03-07-2014 08:38 AM

Re: Microbiology Help [spread plates and biochemical tests on metabolism]
 
Thank you so much for your help on the last question, I went and did some research on PCR after reading this and you answers were a great starting point! :happy:

The first question just said "List two reasons why we spread 0.1ml onto the surface of agar plates when preforming viable counts."
And I came up with the reason that the smaller amount of liquid allowed the agar plates to dry to avoid bacteria running/clumping together. But I couldn't really think of another good reason.

butters 03-07-2014 09:06 AM

Re: Microbiology Help [spread plates and biochemical tests on metabolism]
 
Maybe your answer are combination of two answers.

1. 0.1mL volume was use to allow sufficient coverage to the whole plate without overflowing or under spreading the innoculum while diluting the innoculum
2. Spreading on full plate to avoid two or more bacteria to grow to close together so single colony can be observe after incubation on plate.


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