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-   -   Microtiter plates, bacterial assays and antibiotics (http://www.molecularstation.com/forum/microbiology-forum/87018-microtiter-plates-bacterial-assays-antibiotics.html)

Shane.vontelin.van.breda 12-22-2012 06:24 AM

Microtiter plates, bacterial assays and antibiotics
 
Hey guys, I am busy with an experiment determining MICs for tuberculosis. I am using the MABA method and 96 well microtiter plates. The antibiotic I am using is a cationic cyclic decapeptide with a fatty acid tail. It is amphiphatic. So my question is, what type of plate should I be using for the most accurate result. In know some plates are made from PS so their walls in the wells are hydrophobic. So will this have an effect on my MICs i.e. my antibiotic might be attracted to the walls of the well? But then again maybe my cells as well? So do I get a coated plate or what type of plate? Since I am working with TB I have long incubation periods of up to a week :/ thanks for the input :)


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