Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


E. coli growing on LB+AMP plate, but not in LB+AMP broth

E. coli growing on LB+AMP plate, but not in LB+AMP broth - Microbiology Forum

E. coli growing on LB+AMP plate, but not in LB+AMP broth - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-25-2012, 10:08 PM
Pipette Filler
Points: 13, Level: 1 Points: 13, Level: 1 Points: 13, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question E. coli growing on LB+AMP plate, but not in LB+AMP broth



I am growing up chemically competent E.coli cells for cloning. I have one clone that grows on an LB+Amp plate, but when I inoculate a liquid LB+Amp culture (incubating over night shaking at 37) with this strain I get no growth. I thought that maybe my first Amp plate was faulty so I re-streaked the culture on a new plate and it grew just fine. I tried growing it in liquid culture again to no avail. I have many other strains I have done this with so I'm fairly confident that there is nothing wrong with my protocol. This is the only strain I am having trouble with. Any suggestions?
Reply With Quote
Reply

Tags
amp , ampicillin , broth , coli , growing , growth , lb broth , plate


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Smooth drift gradient across ELISA plate msymeonides ELISA Assay Forum 9 01-05-2012 03:12 PM
What is Gravity? sdr@sdrodrian.com Physics Forum 1 01-27-2008 02:53 PM
Problems with E. Coli Nachiket Vaze Microbiology Forum 3 12-11-2006 04:01 PM
Growing E. coli in 96 well? Yoram Gerchman Protocols and Methods Forum 5 01-13-2004 03:17 AM


All times are GMT. The time now is 04:04 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12656 seconds with 16 queries