Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Microbiology Forum (http://www.molecularstation.com/forum/microbiology-forum/)
-   -   E. coli growing on LB+AMP plate, but not in LB+AMP broth (http://www.molecularstation.com/forum/microbiology-forum/86696-e-coli-growing-lb-amp-plate-but-not-lb-amp-broth.html)

Micro Gal 10-25-2012 10:08 PM

E. coli growing on LB+AMP plate, but not in LB+AMP broth
 
I am growing up chemically competent E.coli cells for cloning. I have one clone that grows on an LB+Amp plate, but when I inoculate a liquid LB+Amp culture (incubating over night shaking at 37) with this strain I get no growth. I thought that maybe my first Amp plate was faulty so I re-streaked the culture on a new plate and it grew just fine. I tried growing it in liquid culture again to no avail. I have many other strains I have done this with so I'm fairly confident that there is nothing wrong with my protocol. This is the only strain I am having trouble with. Any suggestions?


All times are GMT. The time now is 04:56 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09022 seconds with 11 queries