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Unknown Identification

Unknown Identification - Microbiology Forum

Unknown Identification - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 04-06-2012, 02:04 AM
Pipette Filler
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Exclamation Unknown Identification



I am having a bit of trouble identifying an unknown organism, since after reviewing several sources, including the bergey's manual, I am running into conflicting organisms/characteristics. I would appreciate any assistance!

The following describes my organisms and the tests I have run:

Gram Negative
Coccobaccilli
Motility: nonmotile (subjective but almost certain)
FTM: facultative anarobe
Citrate: +
Lysine: +
Glucose: +
Mannitol: -
Lactose: -
Sucrose: -
Maltose: -
Methyl red: +
VP: -
H2S (KIA): -
Indole: -
Urea: -
Litmus milk: purple (confirms nonlactose fermenter)
Gel: -
Nitrate: +
Catalase: +
Oxidase: -/+ (uncertain)

I thought it might have been shigella, but shigella is both citrate AND lysine negative, which clearly conflict. Any help from experienced microbiologists (or anyone with knowledge of these) would be appreciated!!!
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biochemical , identification , microbiology , tests , unknown


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