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Unknown Bacteria Cell Population Question
I have sort of a "dumb" question...or, at least it is starting to feel that way.
It's been quite some time since I have performed bacterial cell counts and I am currently researching an unknown bacteria (a type of Pseudomonas I have yet to determine). I have performed the initial isolation of the species and am now working on growth kinetics so that I may work with it.
I have performed spectrophotometric analysis in order to determine a growth curve for the species. Now I need to figure out an estimate of bacterial cells per mL at varying points of the growth curve. I was told by a colleague that I could take a sample from a point of my growth curve, run a serial dilution, and count via hemocytometer in order to come up with a good estimate of cells at that point. This sounds correct, but does not help me determine cell counts at each point OD was measured...I can't see a clear way to determine this. I don't believe that I need to perform a plate dilution in order to come up with this number.
Does anyone have any input? What would be the best way to determine my cell count without having to laboriously perform a hemocytometer/dilution at each point I pull a sample to run in the spectrophotometer?
Thank you in advance!
Re: Unknown Bacteria Cell Population Question
Thie procedure you mentioned will give you cell counts but is difficult. Why not perform viable counts in parallel with OD measurements?
|bacteria , cell , cell count , dilution , hemocytometer , microbiology , population , question , unknown|
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