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Prepare antibiotic disc

Prepare antibiotic disc - Microbiology Forum

Prepare antibiotic disc - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 12-27-2011, 02:30 AM
Pipette Filler
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Question Prepare antibiotic disc



I was asked to prepare the antibiotic disc myself. I am not so sure about the calculation for the antibiotic stock solution. From the website, I know that the formula is W=(1000/P)xVxC; P=potency, V=volume needed, C = desired concentration.

I have four antibiotics that I need to prepare:-
1) Amoxicillin Trihydrate powder - each gram contains 500mg (dosage: 0.26g/liter drinking water)
2) Kanamycin - each gram contains 550mg (dosage: 0.05g/liter drinking water)
3) Florfenicol - 100mg (dosage: 1cc/1 gallon drinking water)
4) Oxytetracycline 25% + neomycin 25% - each 1000g contains oxytetracycline 250g & neomycin 250g (dosage: 2.2g/liter)


Please help me with the calculation. Thank you.
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Old 03-16-2012, 06:02 PM
Pipette Filler
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Default Re: Prepare antibiotic disc

You can mesure it easy by the microdilution broth '
Start with any start concentration from the net . Hope that will help
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