I have the following problem: why do so many protocols (if not all) demand you to make a "in between culture" (or start culture).
When I am growing bacterial cells, the protocol tells me to inoculate 10ml of broth with a colony from a fresh agar plate.
Then I need to grow this till I reach an OD 0.6 , if so, I need to inoculate 500ml of broth and use these cells.
Now, I wonder: can I simply skip this "in between step" of inoculating 10 ml?
I see no reason why to do this.
Is it perhaps because when I use this in between step the bacteria will grow faster since I add bacteria in the exponential phase in the 500ml?
(although, this in between step takes lots of time too)
(it cant be because I need to make sure the bacteria are expressing the protein/enzyme to cope with the antibiotics, because I pick them from a fresh agar plate..., I can understand it if I would use an old plate or something, but when using a fresh palte
If I could, I would try it out myself (skipping this step) but since my supervisor checks everything I do, I cant risk doing it and he hasnt really given me a good answer to why it is..