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Nitrate Reduction

Nitrate Reduction - Microbiology Forum

Nitrate Reduction - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 12-02-2011, 11:24 AM
Pipette Filler
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Default Nitrate Reduction



Hello, I'm new to this forum, plus I have a question to ask: Nitrate test
How long does the red color have to stay after adding reagents A and B to be called a positive test.

==
Here is what I did in lab:

After incubation, I did the Nitrate test by adding the 2 reagents indicated in my lab manual.
The tube turned red (which should mean (+) for nitrate reduction)
However, the color quickly reverted back to the normal broth color before the addition of reagents A and B.
Every time I tried adding more reagents, the same thing happened: the red color never remained for long;it did turn red, however.

I gave up on the reagents and tried adding some powdered Zinc.
The result was colorless (this should also mean (+) for nitrate reduction)

So, based on my description, would you please tell me if this is a positive test for Nitrate reduction?
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