Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Microbiology Forum (http://www.molecularstation.com/forum/microbiology-forum/)
-   -   Nitrate Reduction (http://www.molecularstation.com/forum/microbiology-forum/84211-nitrate-reduction.html)

TheSuN 12-02-2011 11:24 AM

Nitrate Reduction
 
Hello, I'm new to this forum, plus I have a question to ask: Nitrate test
How long does the red color have to stay after adding reagents A and B to be called a positive test.

==
Here is what I did in lab:

After incubation, I did the Nitrate test by adding the 2 reagents indicated in my lab manual.
The tube turned red (which should mean (+) for nitrate reduction)
However, the color quickly reverted back to the normal broth color before the addition of reagents A and B.
Every time I tried adding more reagents, the same thing happened: the red color never remained for long;it did turn red, however.

I gave up on the reagents and tried adding some powdered Zinc.
The result was colorless (this should also mean (+) for nitrate reduction)

So, based on my description, would you please tell me if this is a positive test for Nitrate reduction?


All times are GMT. The time now is 01:47 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09137 seconds with 11 queries