| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-17-2011, 03:07 PM
| |||||||||||
| |||||||||||
 MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis I have noticed that the MTT growth curves for Mtb strains become less linear after the cultures have been allowed to incubate (while being agitated in a shaking incubator) for more than 15 days. This does not appear to be a problem with the Alamar Blue reagent. Could this be due to the increased concentration of cells in the broth or a result of the cells moving into the stationary phase? please help. thank you  |
|
#2
11-18-2011, 04:10 AM
| ||||||||||||
| ||||||||||||
| Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis uhm... your culturing mycobacterium in flask?! I am just curious what culture medium did you use? |
|
#4
12-08-2011, 07:28 AM
| |||||||||||
| |||||||||||
| Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis I am growing the cultures using 7H9 medium. Growth curves: measure of absorbance at 600 nm versus interval (e.g Day 0, etc.) |
|
#5
03-16-2012, 06:09 PM
| |||||||||||
| |||||||||||
| Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis What if you didnt use shaking incubater maybe this will help |
|
| Tags |
| alamar , assay , blue , mtt , mycobacterium , sensitivity , tuberculosis |
| Thread Tools | |
| Display Modes | |
|
|
Linear Mode
