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MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis

MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis - Microbiology Forum

MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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  #1  
Old 11-17-2011, 04:07 PM
Pipette Filler
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Default MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis



I have noticed that the MTT growth curves for Mtb strains become less linear after the cultures have been allowed to incubate (while being agitated in a shaking incubator) for more than 15 days. This does not appear to be a problem with the Alamar Blue reagent. Could this be due to the increased concentration of cells in the broth or a result of the cells moving into the stationary phase? please help. thank you
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Old 11-18-2011, 05:10 AM
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Default Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis

uhm... your culturing mycobacterium in flask?! I am just curious what culture medium did you use?
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Old 11-23-2011, 12:09 PM
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Default Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis

what is the measure of growth that shows linearity?
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Old 12-08-2011, 08:28 AM
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Default Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis

I am growing the cultures using 7H9 medium.
Growth curves: measure of absorbance at 600 nm versus interval (e.g Day 0, etc.)
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Old 03-16-2012, 06:09 PM
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Default Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis

What if you didnt use shaking incubater maybe this will help
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alamar , assay , blue , mtt , mycobacterium , sensitivity , tuberculosis


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